The study topic focuses on the characterization of a new group of membrane permeabilizing peptides that can create and sustain stable intra-membrane pores and are sensitive to molecular environmental properties such as pH. Membranes play a critical role in various processes such as the development of eukaryotic life forms and the emergence of complex brain signaling networks. They have a crucial barrier property that allows selective passage of molecules, and recent research has shown that disrupting the membrane can lead to increased druggable targets and therapeutic utility. The primary aim of this research is to present a group of membrane permeabilizing peptides that are sensitive to pH and have the capacity to create and sustain membrane pores. A comprehensive peptide library will be used to identify these molecules through high-throughput orthogonal assays. The research will also investigate the behavior of the membrane permeabilizing peptides under different molecular parameters such as lipid-peptide ratio and amino acid sequence. Understanding the properties and behavior of these peptides can have significant clinical and biotechnological applications, such as biosensors and therapeutic treatments.

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